Figure 6
Confirmation of bidirectional, optical communication in the mouse brain.
Using in utero electroporation, both ChR2-Venus and O-GECO1 were delivered to the neural cells in the mouse brain. (a–c) The postnatal day 14 mouse brain was examined for expression of green and red fluorescent proteins. Fluorescence images were taken using a stereo fluorescence microscope. (d) shows the experimental setup for application of the device. (e,f) Bright-field and fluorescence images were taken using a stereo fluorescence microscope. Red arrow shows the presence of fluorescent cells. (g) Fluorescence image taken using the sensor. The numbers in the image indicate ROIs. Temporal changes in fluorescence intensity when the brain was stimulated with local blue light are shown as pseudo-colored images. Values from the sequential ROIs are plotted in the graph (h). ROIs 4 and 5 do not include positive cells and were considered as baseline and negative control, respectively.
