Figure 2 | Scientific Reports

Figure 2

From: Three-dimensional spherical spatial boundary conditions differentially regulate osteogenic differentiation of mesenchymal stromal cells

Figure 2

Cell viability of MSCs in the 3D scaffolds (Groups I, II, III and IV) and on the 2D flat gel.

(a) Fluorescence micrographs of live and dead MSCs in the 3D scaffolds or on the 2D flat gel at 1 day of culturing. Live cells were stained with calcein-AM (green) and dead cells were stained by ethidium homodimer-1 (red). (b) Analysis and quantification of cell viability from five random microscopic fields at 100 × magnification were displayed as the percentage of live cells to total cell numbers. Data were represented as mean ± S.D., n = 5. N.S., no significance.

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