Table 1 Primer sequences and probes from Universal ProbeLibrary used in qPCR analysis.

From: Three-dimensional spherical spatial boundary conditions differentially regulate osteogenic differentiation of mesenchymal stromal cells

Gene name

Primer sequences

Probe number

Runx2

5′-gtgcctaggcgcatttca-3′

29

5′-gctcttcttactgagagtggaagg-3′

Osterix

5′-taacctgatggggtcatggt-3′

43

5′-gactgcagagcaggttcctc-3′

ColI A1

5′-cccctggaaagaatggagat-3′

60

5′-aatcctcgagcaccctgag-3′

Osteonectin

5′-gtgcagaggaaaccgaagag-3′

77

5′-tgtttgcagtggtggttctg-3′

Vinculin

5′-gatgaagctcgcaaatggtc-3′

28

5′-tctgcctcagctacaacacct-3′

α1 integrin

5′-aattggctctagtcaccattgtt-3′

14

5′-caaatgaagctgctgactggt-3′

α2 integrin

5′-tcgtgcacagttttgaagatg-3′

7

5′-tggaacacttcctgttgttacc-3′

α5 integrin

5′-cccattgaatttgacagcaa-3′

55

5′-tgcaaggacttgtactccaca-3′

α11 integrin

5′-gaggctgacgtcctcttcac-3′

66

5′-gttgggcttgacctcgtagt-3′

β1 integrin

5′-cgatgccatcatgcaagt-3′

65

5′-acaccagcagccgtgtaac-3′

GAPDH

5′-agccacatcgctcagacac-3′

60

5′-gcccaatacgaccaaatcc-3′

  1. Abbreviation: Runx2, runt-related transcription factor 2; ColI A1, Type I collagen alpha1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
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