Figure 5: Effects of centrifugation and blood plasma.
From: A DNA aptamer recognising a malaria protein biomarker can function as part of a DNA origami assembly
(a–c) Centrifugation of modified origamis constructed using 12 aptamers in the presence of PfLDH resulted in a significant decrease in the amount of unbound protein while aptamer-bound protein remained. Centrifugation also promoted aggregation of origami structures, apparently via protein-protein interactions resulting in (b) dimerisation or (c) larger aggregates (indicated by arrows). (d) Presence of blood plasma has little effect on AM-DNA origami integrity or protein binding ability. Linear arrangements of PfLDH are clearly visible and some aggregation occurs due to protein-protein interaction as a result of centrifugation.
