Figure 4

Kinetics of non-photochemical quenching (NPQ) rise in dark-adapted cells.

Panel (a–e) NPQ induction and relaxation kinetics measured on WT (black) npq4 (red) and npq4 lhcsr1 (blue) mutants. The NPQ kinetics were obtained by illuminating whole cells with actinic light (1500?µmol photons m^-2?s^-1) for 8?minutes followed by dark recovery. Panel (f) Maximum level of NPQ after 8?min of light treatment. Statistical significant differences were obtained between WT, npq4 and npq4 lhcsr1 according to two-tailed non parametric Kruskal-Wallis test multiple comparison (n?=?5, a?=?0.05 for all condition, p values of 0.0019 for both Light A,B respectively). Wilcoxon-Mann-Whitney test was applied for two samples comparison obtaining statistically significant differences in all growth conditions comparing WT to npq4 or npq4 lhcsr1 or even comparing npq4 to npq4 lhcsr1 (n?=?5, a?=?0.05, p?=?0.009 for all conditions).