Figure 1 | Scientific Reports

Figure 1

From: Three-Dimensional Characterization of Mechanical Interactions between Endothelial Cells and Extracellular Matrix during Angiogenic Sprouting

Figure 1

Images of a sprout and beads contained within the ECM.

Immunofluorescence staining of the sprout with CMFDA was performed nearly 1 hour before imaging. To reduce the potential toxicity of the dyes, the cell channel and the opposite media channel of the devices were rinsed by delivering the media with the VEGF gradient into both channels after 30 minutes of staining had been completed. The images were acquired using an Olympus FV1200 confocal microscope with a 60× oil immersion objective. (A–C) collectively show a sprout (green) invading the ECM (with collagen fibers and beads displayed in cyan.) (D,E) solely show 3D views of the sprout in which the lamellipodia experiencing retraction were marked with an arrowhead. (G) solely shows the beads introduced in the ECM. A combined image is shown in (H).

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