Figure 5
Experimental observations of the “retraction-related push” and “release” behavior of the tip cell (in green ellipse).
Images were produced the same way that those of Fig. 4 were generated. For the “retraction-related push,” the involved beads (in ellipses) located at the back of the retracting lamellipodium were pushed away from the lamellipodium in a direction that was consistent with that of the major axes of those ellipses, which approximates the lamellipodium’s retraction direction (shown by an arrow). The beads (in orange circles) linked to the “release” behavior of the retracting lamellipodium were radially displaced away from the lamellipodium. Among them, those (e.g., the one inside the largest circle) closer to the lamellipodium were more significantly displaced than the others in the same group. It should be noted that the highlighted beads were not in the same plane (which was observable prior to focus stacking), indicating the presence of out-of-plane action.
