Figure 2
From: Detection and delineation of oral cancer with a PARP1 targeted optical imaging agent
Immunofluorescence staining of PARP1 in human oral cancer squamous cell carcinoma xenografts (OSCC).
(a) H&E staining with corresponding PARP1 immunofluorescence microscopy of OSCC xenografts. (b) H&E staining with corresponding PARP1 immunofluorescence microscopy of normal mouse tongue. Blue = cell nuclei stained with DAPI; red = PARP1 staining. (c) Quantification of the PARP1-positive area in relation to the whole tissue area and average intensity of PARP1 staining in all nuclei, for various xenograft and normal mouse control tissues. The PARP1-positive tissue area was determined by performing a thresholding on red (PARP1) and green (autofluorescence of total tissue, not displayed here) areas and the relative PARP1-positive area was calculated by dividing the PARP1-positive area by the total tissue area. Values display means ± SD of n = 10 values per tumor/organ. See Supplementary Fig. S3 for images of trachea and thigh muscle, as well as images obtained with a staining control.
