Figure 3: Detection of P3N-ALT accumulated in ClYVV-infected plant tissues. | Scientific Reports

Figure 3: Detection of P3N-ALT accumulated in ClYVV-infected plant tissues.

From: Truncated yet functional viral protein produced via RNA polymerase slippage implies underestimated coding capacity of RNA viruses

Figure 3

(a) Schematic diagram of Cl30 and RB derivatives carrying a GFP gene. (b-d) Detection of GFP fluorescence and P3N-ALT in systemically infected tissues. Leaf (b), stem (c) and flower (d) tissues were harvested at 5, 10, and 24 days post-inoculation, respectively. The infected areas were confirmed by virus-derived GFP fluorescence (upper panels). P3N-ALT was detected by western blotting (lower panels), using a polyclonal antibody raised against the N-terminal region of P3. Coomassie brilliant blue (CBB)-stained gel images are shown at the bottom of each western blot panel as a loading control. The position of P3N-ALT is marked with an arrowhead. The asterisks denote the background signals derived from non-specific cross-reaction of the antiserum. Positions of molecular mass markers (kDa) are indicated on the left side of each panel. The analysis was repeated three times, and typical images are shown.

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