Figure 4: Functional analysis of P3N-ALT supplied in trans in ClYVV infection. | Scientific Reports

Figure 4: Functional analysis of P3N-ALT supplied in trans in ClYVV infection.

From: Truncated yet functional viral protein produced via RNA polymerase slippage implies underestimated coding capacity of RNA viruses

Figure 4

(a) Schematic diagram of Cl-P3ΔARFPs. The G2A6 motif with the downstream sequence is indicated below each diagram. The mutated nucleotides in Cl-P3ΔARFPs are shown in lower case red letters. The natural stop codon for the alt ORF (+1 stop) and the introduced stop codon for the pipo ORF (−1 stop) are italicized and shaded. (b) Requirement of P3N-ALT and P3N-PIPO for efficient cell-to-cell movement of ClYVV. Pea leaves were biolistically co-inoculated with Cl-P3ΔARFPs and the white clover mosaic virus vectors (WCl) expressing P3N-ALT (WCl/P3N-ALT), P3N-PIPO (WCl/P3N-PIPO), and both P3N-ALT and P3N-PIPO [WCl/P3N-PIPO(ALT–1, P3+1)]. Cell-to-cell movement was monitored by GFP fluorescence of Cl-P3ΔARFPs. GFP-expressing lesions at 1 or 3 days post-inoculation (dpi) are shown. The biolistic inoculations of 6 leaves with each plasmid or mixture of plasmids were repeated twice, and typical images are shown. Scale bar =50 μm.

Back to article page