Figure 4: Mitochondria in a HEK293-T cell labeled with DAKAP-Dronpa visualized in Epi-illumination mode.
From: Sparse deconvolution of high-density super-resolution images
Experiments were performed on an Olympus cellTIRF microscope equipped with an additional EMCCD camera. Images (1,000 frames) were recorded with a constant 488 nm laser excitation and a camera exposure time of 30 ms: (a) Average live-cell image and SPIDER reconstruction of the hollow mitochondria structures (averaging of density estimations over 1,000 frames); (b) Average live-cell image and SPIDER reconstruction (averaging of density estimations over 1,000 frames) of the region indicated by the box in (a), sized 4 μm by 8 μm. Note the blurred and thickened membranes which indicate movement of the cell during acquisition (see Supplementary Fig. S4).
