Figure 5
From: Straightforward and sensitive RT-qPCR based gene expression analysis of FFPE samples
Overview of non-normalized gene expression (Cq values) in preamplified and non-preamplified samples processed with gene-specific RT and whole transcriptome RT.
(a) Unsupervised hierarchical clustering of Cq values for 48 genes in 10 HEK-293T FFPE cell pellets and 8 no template controls (NTCs). Distance measure = Manhattan, clustering method = Ward. (b) Boxplot analysis for comparison of gene expression levels in preamplified samples (PA), non-preamplified gene-specific RT samples (GSP), non-preamplified whole transcriptome RT samples (WT) and no template controls (NTC), shown for Qiagen (Q) and Epicentre (E) RNA isolates. (*) All differences are significant (two-tailed paired t-test based, P < 0.001), with exception of differences within the NTC boxplot group. Whiskers extend to data points that are less than 1.5× the inter quartile range away from 1st/3rd quartile. Horizontal line depicts median value.
