Table 2 Overview of RT-qPCR conditions performed on HEK-293T FFPE cell pellets and no template control samples.

From: Straightforward and sensitive RT-qPCR based gene expression analysis of FFPE samples

cell line

whole transcriptome RT*

gene-specific RT*

gene-specific PA**

Qiagen

Epicentre

HEK

+

  

+

 

HEK

+

  

+

 

HEK

+

   

+

HEK

+

   

+

HEK

 

+

 

+

 

HEK

 

+

 

+

 

HEK

 

+

  

+

HEK

 

+

  

+

HEK

+

 

+

 

+

HEK

+

 

+

 

+

PA NTC

+

 

+

  

PA + MS2 NTC

+

 

+

  

WT NTC

+

    

WT NTC

+

    

GSP NTC

 

+

   

GSP NTC

 

+

   

H2O NTC***

     

H2O NTC***

     
  1. RT = reverse transcription; WT = whole transcriptome; GSP = gene specific; PA = preamplification; Q = Qiagen; E = Epicentre.
  2. HEK = HEK-293T FFPE cell pellet RNA; MS2 = MS2 phage carrier RNA.
  3. (*) 300 ng RNA was used for whole transcriptome and gene-specific RT reactions; (**) 50 ng RNA was used for whole transcriptome RT reactions intended for preamplification; (***) H2O NTC samples were only used for 5 μl qPCR reactions.
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