Figure 6: Down-regulation of TbMTase37 leads to a reduction in the steady-state levels of srRNA 4 and 5S rRNAs. | Scientific Reports

Figure 6: Down-regulation of TbMTase37 leads to a reduction in the steady-state levels of srRNA 4 and 5S rRNAs.

From: A tRNA methyltransferase paralog is important for ribosome stability and cell division in Trypanosoma brucei

Figure 6

(a) RNA isolated from the sucrose gradient fraction as in Fig. 5 were analyzed by Northern hybridization with oligonucleotide probes specific for each small ribosomal RNA fragment. Cropped panels are the signals of expected rRNA size and were used in quantification. Gels were loaded with equal A260 units. Fraction numbers are as in the previous figure. (b) Quantification of the signals in (a), the signal of each rRNA in each fraction was first normalized as a percentage of total probe intensity across all fractions to exclude oligo hybridization differences. Relative signals were used to calculate the signal ratio of wild type to RNAi. Calculations were made as the percentage of signal for each rRNA in each fraction divided by the sum of all probe percentages in that fraction and multiplied by 6 (the total number of rRNA bands). The dashed line across each graph represents the expected 1:1 stoichiometry for all rRNAs within a given fraction.

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