Figure 1: SILAC-based comparative proteomic analysis of fatty acid-treated myoblasts.

(A) Flowcharts of forward SILAC and reverse SILAC labeling combined with LC-MS/MS for the comparative analysis of protein expression in C2C12 myoblasts upon PA and PA + OA treatment(R0/K0: normal isotopes, K4/R6: [²H₄]lysine/[¹³C₆] arginine, K8/R10: [¹³C₆,¹⁵N₂]lysine/[¹³C₆,¹⁵N₄] arginine). (B) Quantitation overlap of the quantified proteins in the two forward and one reverse SILAC labeling experiments (F1&F2: forward experiment, R1: reverse experiment). (C) The distribution of expression ratios for the quantified proteins, including those quantified in only one set of SILAC labeling experiment, and the mean protein expression ratios were used for the data quantified in at least two sets). (D) (STRING database mapping the interaction of the differentially expressed proteins upon the treatment of PA and PA + OA. These proteins form several apparent interaction groups exerting diverse biological functions including ER stress, lipid metabolism, immune response, gene transcription and protein synthesis.