Figure 3: Long-term culture experiments.
From: Long-term ex vivo maintenance of testis tissues producing fertile sperm in a microfluidic device
(A) A testis tissue fragment, from Acr-Gfp neonates, at 0.5 dpp, was cultured in MF, and GFP expression lasted throughout the period. (B) In the same tissue, by magnification, GFP-positive acrosomes were observed intermittently even in the late culture period. (C,D) MF and AG were compared based on the frequency of tissue samples expressing GFP grade 1 or above (C) and over 2 (D). (E) The average GFP-grade transition was compared in MF and AG. Scale bars: 500 μm (A), 10 μm (B).
