Figure 1: Osteoclasts differentiation and specific genes expression change at different stages.
From: Changing expression profiles of lncRNAs, mRNAs, circRNAs and miRNAs during osteoclastogenesis
(A) Representative images of TRAP stain of osteoclasts cultured from RAW264.7 cells induced with RANKL (100 ng/mL) and M-CSF (50 ng/mL) for 0 h, 24 h, 72 h and 96 h. The upper scale bar represents 200 μm and the lower scale bar represents 50 μm. Multinucleated cells were highlighted by black arrows. (B) Quantitative analysis of TRAP positive cell proportion of at each stage during the differentiation. (C) Proportion of TRAP positive cells with more than three nuclei (three included) in total TRAP positive cells at each differentiation stage. (D) Osteoclasts specific and fusogenic genes expression level alterations from 0 h (monocytes) to 96 h (mature osteoclasts).The data in the figures represent the averages ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001 based on one-way ANOVA.
