Figure 1

Vimentin is downregulated by live H37Rv.

(a) Macrophage monolayers were infected with live H37Rv and heat-killed H37Rv and uninfected macrophage was set as control. 2DE was done after 24 h post infection and visualized by Coomassie Blue. The arrow points to the spot picked and later identified as vimentin by MALDI-TOF-TOF. The experiment is a representative of 2 independent experiments with similar results. (b) qRT-PCR was done using the RNA isolated from uninfected macrophages and macrophages infected with heat-killed or live M. tuberculosis H37Rv, 12 h after infection and setting GAPDH as reference gene. Expression of vimentin in live H37Rv-infected macrophages is decreased when compared to heat-killed H37Rv-infected macrophages or uninfected macrophages. Values are expressed as mean ± SE, n = 3, *Mean difference is significant at 0.05 level. (c) Macrophage monolayers were infected with live H37Rv, heat-killed H37Rv and H37Ra. After 12 h of infection different samples were processed for Western blotting. β-tubulin was used as loading control. The expression of vimentin is decreased in live H37Rv-infected macrophages when compared to heat-killed H37Rv infected macrophages, H37Ra-infected macrophages and uninfected macrophages. Values are expressed as mean ± SE, n = 3. *Mean difference is significant at 0.05 level.