Figure 3 | Scientific Reports

Figure 3

From: Constructing modular and universal single molecule tension sensor using protein G to study mechano-sensitive receptors

Figure 3

DLD-1 cells specifically adhere on Ecad-Fc:ProG-TGT coated surface.

(A) Cells did not adhere on ProG-TGT grafted surface (no Ecad-Fc). Middle panel shows a fluorescence image of Cy3 conjugated to ProG-TGT. The bright area marks the ProG-TGT coated region. Bottom panel shows a phase contrast image of the same area. (B) Cells specifically adhered on Ecad-Fc:ProG-TGT coated surface marked by strong fluorescence. Fluorescence loss (gray patches in TGT grafted area) underneath the cells suggests that cells caused TGT rupture. (C) Under higher magnification, it is evident that gray patches of fluorescence loss are co-localized with cells, confirming that TGTs were ruptured by DLD-1 cells.

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