Figure 4
Ang-(1–7)/ACE2 ameliorated hepatic steatosis in FFA-induced HepG2 cells.
Cells were exposed to 2 mM of FFA (2:1, oleate: palmitate) for 24 h. FFA-induced hepatic steatosis in HepG2 cell treated with Ang-(1–7) or A779. (A) Analyze lipid accumulation by Oil Red O staining. (B) Intracellular lipids were stained with Nile red and the fluorescence intensity of Nile red was measured by flow cytometry. (C) Intracellular lipids content analysis following ACE2 overexpression. (D) Relative protein levels of ACCα, SREBP-1c, LXRα, FAS, UCP-2 and AdipoR1 in ACE2-overexpressing HepG2 cells and FFA-induced HepG2 cells. (E) Relative protein levels of ACCα, SREBP-1c, LXRα and FAS in API-2 or MK-2206-treated ACE2-overexpressing HepG2 cells. Cells were treated with API-2 (20 μM) and MK-2206 (10 μM) for 24 h. The data are presented as the mean ± SD of n = 3 independent experiments in HepG2 cells. *P < 0.05 and **P < 0.01 versus GFP group by one-way ANOVA; #P < 0.05 versus ACE2 group by one-way ANOVA.
