Figure 4
From: Oscillating primary transcripts harbor miRNAs with circadian functions
Transcriptome profile of miR-378 over-expression in mouse liver.
(a) Heatmap of all genes affected by miR-378 over-expression. The genes are clustered into two groups based on their expression patterns. High (red) and low (yellow) expression values as Z-scored normalized ratios are indicated in the color scale bar at the bottom. Black/white bars indicate the circadian day (white) and night (black) of the consecutive circadian cycles. (b) The number of overlapping genes between under-expressed genes in Ad-378 comparing to Ad-null (green circle) and miR-378-3p targets from Ruckerl et al.’s data (purple circle). Enrichment p-value was determined by Fisher’s exact test. (c) Enrichment of circadian transcripts in under-expressed genes upon miR-378 over-expression. Enrichment p-value was determined by proportion test. (d) Comparison of the circadian peak times in our data with the peak times from mouse liver circadian database. The red curve represents the circadian peak time distribution for genes over-expressed at CT22 in our data. The blue curve represents the circadian peak time distribution for genes over-expressed at CT10 in our data. (e) Heatmap of the circadian oscillating genes affected by miR-378 over-expression. The transcripts are clustered into four groups based on their expression patterns. High (red) and low (yellow) expression values as Z-scored normalized ratios are indicated in the color scale bar at the bottom. Black/white bars indicate the circadian day (white) and night (black) of the consecutive circadian cycles.
