Figure 4
PE11 has esterase activity.
(a) Lipid hydrolyzing activity of PE11 was determined by measuring the release of para-nitrophenol (pNP) upon incubation of 20 μg of cell lysate prepared from either Msmeg-pVV or Msmeg-PE11 with p-nitrophenyl acetate (C2), p-nitrophenyl butyrate (C4), p-nitrophenyloctanoate (C8), p-nitrophenyldodecanoate (C12), p-nitrophenylmyristate (C14), p-nitrophenylpalmitate (C16) and p-nitrophenyl stearate (C18) at 37 °C and pH 7.0 for 10 min. The release of para-nitrophenol was measured spectrophotometrically at 405 nm. (b) Also, lipolytic activity of PE11 was monitored by cleavage of polyoxyethylene sorbitan monolaurate (Tween 20) and polyoxyethylene sorbitan monooleate (Tween 80) at pH 7.0. Data are representative of mean ± SD of three different experiments.
