Figure 5
Modulation of total, polar and glycolipids in Msmeg-PE11.
Quantification (% weight) of (a) total lipids, (b) polar and apolar lipids extracted from the Msmeg-pVV or Msmeg-PE11 wet pellets (equal weights), with chloroform-methanol (2:1, vol/vol). (c) Total glycolipid analysis of Msmeg-pVV or Msmeg-PE11 by analytical TLC. Methanol: Ammonium hydroxide (80:20, vol/vol) was used as mobile phase and the glycolipids were visualized by α-naphthol/sulfuric acid staining (d) Mycolic acid containing glycolipids were separated from the pool of total polar lipids using Chloroform:Methanol:Ammonium hydroxide (80:20:2, vol/vol/vol) as mobile phase. These glycolipids were identified by Iodine vaporization followed by charring with 5% phosphomolybdic acid.
