Figure 2: Production of chimeric HEV-LP possessing monovalent foreign epitope. | Scientific Reports

Figure 2: Production of chimeric HEV-LP possessing monovalent foreign epitope.

From: Production of hepatitis E virus-like particles presenting multiple foreign epitopes by co-infection of recombinant baculoviruses

Figure 2

(A) CBB staining and immunoblotting of the culture supernatants of Tn5 cells infected with AcHEV (light brown), AcMyc-HEV (blue), AcHA-HEV (red) or AcFLAG-HEV (green). Asterisk and arrow in the CBB staining gel indicate bovine serum albumin and recombinant HEV capsids, respectively. (B) Sucrose density gradient fractionation of the culture supernatants of cells infected with the recombinant viruses. Recombinant capsid proteins were indicated in the left of the panels. Each fractions were subjected to CBB staining and immunoblotting after SDS-PAGE. (C) Electron microscopic observation of the middle fractions of the sucrose density gradient. HEV-LP were imaged by negative staining. Bars indicate 100 nm.

Back to article page