Figure 4: Incorporation of multiple recombinant capsids into HEV-LP.

HEV-LP recovered from insect cells co-infected with recombinant viruses were immunoprecipitated by either anti-FLAG (A), -myc (B), or -HA (C) antibody and the immunoprecipitates were further examined by immunoblotting by indicated antibodies. In put of each HEV-LP stained by CBB is indicated in the bottom. (D) Immunoelectron microscopic observation of the chimeric HEV-LP consisting of the myc- and FLAG-tagged capsids and of the myc- and HA-tagged capsids were stained with anti-myc (left) and anti-HA antibody (right), respectively. Bars indicate 100 nm.