Figure 2 | Scientific Reports

Figure 2

From: Isolation of a recombinant antibody specific for a surface marker of the corneal endothelium by phage display

Figure 2

Screening of individual clones from enriched libraries ETC-H1-C1M2 (a) and ETC-H1-C3 (b) by monoclonal soluble scFv ELISA. hCECs and stromal fibroblasts were seeded in 96 well plates and incubated with soluble scFv. Specific binding was detected by c-myc antibody conjugated to horseradish peroxidase. OD readings of the different clones generated on hCECs were sorted from highest to lowest and plotted with the corresponding signals on fibroblasts.

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