Figure 3 | Scientific Reports

Figure 3

From: Isolation of a recombinant antibody specific for a surface marker of the corneal endothelium by phage display

Figure 3

Immunofluorescence microscopy on hCECs and stromal fibroblasts.

Cell cultures of hCECs (95 K/well) and stromal fibroblasts (300 k/well) were seeded onto a 12-well multi slide, fixed with formaldehyde and stained with C1M2-2-B8 scFv-AP fusion (100 μg/ml) or AP protein without scFv (100 μg/ml). Specific binding was detected by Vector Red alkaline phosphatase substrate. Nuclei of cells were stained by DAPI.

Back to article page