Figure 8 | Scientific Reports

Figure 8

From: Isolation of a recombinant antibody specific for a surface marker of the corneal endothelium by phage display

Figure 8

Identification of IgG-B8 antigen target.

(a) hCEC total lysate probed with IgG-B8 under non-reducing (NR) and reducing (R) conditions. (b) IP on hCEC total cell lysate using the IgG-B8 enriched a protein (arrow) which was excised and analysed by LC/MS-MS. This band was not enriched when IgG-B8 was omitted in the experiment (−ve). (c) IgG-B8 IP samples were probed with IgG-B8 and a commercial anti-CD166 antibody.

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