Figure 7 | Scientific Reports

Figure 7

From: Using mutagenesis to explore conserved residues in the RNA-binding groove of influenza A virus nucleoprotein for antiviral drug development

Figure 7

(a) The M1 viral RNA synthesis in the influenza virus-infected cells upon H7 treatment. Total cellular RNA was isolated and subjected to RT-PCR and Q-PCR for the detection of H1N1 viral RNA and GAPDH RNA. (b) Effects of (E,E)-1,7-bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione (H7) on NP and M1 viral proteins expression. Cellular extracts were isolated and subjected to western blot analysis for the measurement of viral protein α-NP, α-M1 and α -tubulin. A549 cells were mock-infected or infected with H1N1 for 8 h. Various concentrations of H7 were added as indicated at T2 time point. (c) The effect of H7 on the cytotoxicity. Cell cytotoxicity of H7 was determined by MTS assay.

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