Table 1 The RNA binding kinetic analysis of H1N1 wild-type and mutant nucleocapsid proteins.

From: Using mutagenesis to explore conserved residues in the RNA-binding groove of influenza A virus nucleoprotein for antiviral drug development

Sample

ka (M−1 s−1 × 104)

kd(s−1 × 10−4)

KD(nM)

WT

1.83 ± 0.22

1.52 ± 0.19

8.27 ± 1.43

Y148F

1.30 ± 0.07

2.07 ± 0.19

15.87 ± 1.74

Y148S

1.21 ± 0.04

3.97 ± 0.11

32.69 ± 1.30

Y148R

1.82 ± 0.10

1.67 ± 0.66

9.14 ± 3.65

Y148A

1.09 ± 0.17

7.31 ± 1.49

66.71 ± 17.19

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