Table 3 The RNA binding kinetic analysis of H1N1 wild-type nucleocapsid protein in the presence of H1, H3, H5, or H7 compounds.

From: Using mutagenesis to explore conserved residues in the RNA-binding groove of influenza A virus nucleoprotein for antiviral drug development

Sample

kd(S–1 × 10–4)

ka(M–1 S–1 × 104)

KD(nM)

WT

1.52 ± 0.19

1.83 ± 0.22

8.27 ± 1.43

H1

1.73 ± 0.24

1.66 ± 0.32

10.42 ± 2.48

H3

1.72 ± 0.41

1.78 ± 0.14

9.66 ± 2.43

H5

1.86 ± 0.31

1.59 ± 0.17

11.69 ± 2.32

H7

1.74 ± 0.18

0.90 ± 0.09

19.33 ± 2.78

  1. H1: 3,3′-Methylenebis(4-hydroxycoumarin); H3: N-2-,N-2-Dimethyl-N-1-(6-oxo-5,6-dihydrophenanthridin-2-yl)glycinamide; H5: N,N-Bis(2,5-dihydroxybenzylidene)ethylenediamine; H7: (E,E) -1,7-bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione.
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