Figure 2: The binding affinity and specificity assays. | Scientific Reports

Figure 2: The binding affinity and specificity assays.

From: Graphene oxide-assisted non-immobilized SELEX of okdaic acid aptamer and the analytical application of aptasensor

Figure 2

(a) Binding saturation curves of selected aptamer candidates to OA. The Kd values were determined by a nonlinear regression analysis using GraphPad Prism 5.0. (b) Characterization of the specificity of aptamer candidates. (c) Binding saturation curve and Kd value of truncated aptamer OA27-1. (d) Characterization of the specificity of truncated aptamer OA27-1. ΔF = F −F0, where F and F0 are the fluorescence intensities at 520 nm in the presence and absence of target, respectively. Relative fluorescence ratio = ΔFt/ΔFOA, where ΔFt and ΔFOA are the ΔF values of each target and Okadaic acid, respectively.

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