Figure 4: Optimization of assay conditions.
(a) Reduced absorbance of avidin under different coating conditions, including avidin concentration and incubation time. (b) Absorbance at 550 nm of the reaction of different concentrations of gold trichloric acid with gradient concentrations of H2O2, and a photograph showing the color variation of the microplate. (c) Absorbance at 550 nm of the negative experiments using different dilution ratios of avidin-catalase conjugate, and photograph showing the color change of the microplate. (d) A550 and ΔA550 of the checkerboard experiment with different concentrations of OA27-1 and COA27, and a photograph showing the color change of the microplate.
