Figure 1: Summarized workflow of NATpipe. | Scientific Reports

Figure 1: Summarized workflow of NATpipe.

From: NATpipe: an integrative pipeline for systematical discovery of natural antisense transcripts (NATs) and phase-distributed nat-siRNAs from de novo assembled transcriptomes

Figure 1

(A) Two functional modules “NAT prediction” and “Search for phased sRNAs” were integrated into the pipeline. The first module requires de novo assembled transcripts as the input, and the second module requires small RNA (sRNA) HTS data (at least) and degradome HTS data (would be best if available). The parameters in blue color are adjustable. (B) Illustration of the phase-distributed sRNAs identified within the perfectly annealed regions (>80 bp) of a NAT pair. As an example, eight sRNAs with consistent sequence length were assigned to four phases (four sRNAs on each strand). Each phased sRNA duplex (sRNA1/sRNA8, sRNA2/sRNA7, sRNA3/sRNA6 and sRNA4/sRNA5) possesses 2-nt overhangs at their 3′ ends (dashed boxes just indicate two 2-nt overhangs for example).

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