Figure 1
From: Mechanisms and consequences of ATMIN repression in hypoxic conditions: roles for p53 and HIF-1
ATMIN loss does not impair ATM activation in hypoxia.
(A) RKO cells were transfected with either non-targeting scrambled siRNA or ATMIN-specific siRNA. 24 h post transfection, cells were exposed for up to 3 h of hypoxia (<0.1% O2). (B) Colony survival assay of RKO cells treated with 10 μM KU-55933, exposed to 6 h hypoxia (<0.1% O2) and irradiated with 0–6 Gy. Post treatment, cells were allowed to form colonies for 8 days under normal tissue culture conditions. Mean and standard deviation (SD) from 3 independent experiments is shown. (C) RKO cells transfected with either scrambled (Scr) siRNA or ATMIN siRNA were exposed to 6 h of hypoxia treatment (<0.1% O2) or left at normoxia (21% O2), followed by irradiation (4 Gy). Post radiation, cells were reoxygenated and kept under normal tissue culture condition to allow colony formation. (D) Colony survival assay of RKO cells transfected with ATMIN siRNA, exposed for up to 36 h of hypoxia (<0.1% O2). For all colony formation data mean surviving fractions are shown ± SD from three independent experiments.
