Figure 2
From: Mechanisms and consequences of ATMIN repression in hypoxic conditions: roles for p53 and HIF-1
ATMIN expression in hypoxia decreases in an oxygen dependent manner through HIF-1α and p53-dependent pathways.
(A–C) RKO (A), HCT116 (B) and RPE1-hTERT (C) cells were exposed to hypoxia (<0.1% O2) for the times indicated and western blot analysis was carried out using the antibodies indicated (β-actin was used as a loading control). (D) RKO cells were treated as indicated for a period of 16 h. The hypoxia mimetic drugs were used as follows; 100 μM DFO and 150 μM CoCl2. Western blots using the antibodies indicated are shown. (E) RKO cells were exposed to DMSO, 1 mM Hu or 5 μg/ml APH for the indicated amount of time and western blot analysis was carried out for ATMIN, RPA 32 and β-actin (loading control). (F) HCT116 p53−/− cells were transfected with either HIF-1α siRNA or Scr siRNA and HCT116 p53+/+ cells were transfected with Scr siRNA. Cells were exposed to hypoxia (<0.1% O2) for the times indicated and protein lysates analyzed by western blotting with the antibodies shown.
