Figure 3
From: Mechanisms and consequences of ATMIN repression in hypoxic conditions: roles for p53 and HIF-1
Hypoxia represses ATMIN without increasing degradation (A–C) RKO (A), HCT116 (B) and U-87 (C) cells were exposed to hypoxia (<0.1% O2) for the times indicated and ATMIN mRNA expression was analyzed by qPCR in relation to 18S (reference gene). The mean mRNA expression ± SD from three independent experiments is shown. (D) RKO cells were treated with DMSO or 5 μM MG-132 under normoxic (21% O2) or hypoxic (<0.1% O2) conditions for the times indicated and ATMIN levels were analyzed by western blotting. (E) RKO cells were treated with 25 μg/mL cycloheximide under normoxic (21% O2) or hypoxic (<0.1% O2) conditions for the times indicated. ATMIN protein expression was measured by western blotting and quantified in relation to β-actin levels. The mean ATMIN protein expression ± SD from three independent experiments is shown.
