Figure 6: Characterisation of platelet activation.
From: Microfluidic model of the platelet-generating organ: beyond bone marrow biomimetics
(a) Two-colour flow cytometry analysis of platelet receptors without or with activation with TRAP. Dot plots within the platelet gate indicate the population of PAC1 positive, CD42b+ platelets in the non stimulated (upper panels) and TRAP stimulated samples (lower panels) produced in the microfluidic device (left panels) vs control (right panels). (b) Quantification corresponding to the results presented in panel (a) (mean values for 4 independent experiments). (c) Aggregation in the presence of fibrinogen and CaCl2. Platelet aggregates are observed before (upper panels) or after (lower panels) activation with TRAP. The black arrow indicates a large aggregate visible in the sample collected at the exit of the fluidic device (lower left panel). Fragments recovered in the control samples do not aggregate in the presence of the agonist peptide (lower right panel). The scale bar represents 10 
m.
