Figure 2
From: Regulation of Transcription Factor Yin Yang 1 by SET7/9-mediated Lysine Methylation
YY1 is methylated by SET7/9 in cultured cells.
(A,B) Increased amount of short peptides containing unmodified (K173 or K411) or mono-methylated K173 or K411 (K173me1 or K411me1) were prepared for dot blot assay, followed by immunoblotting (IB) using anti-YY1K173me1 (A) or anti-YY1K411me1 (B) antibody as indicated. (C) HEK293T cells transfected with control vector or vectors expressing Flag-tagged wild type (wt) or mutant YY1 (K173R or K411R) were subjected to IB with anti-YY1K173me1, anti-YY1K411me1 or anti-Flag antibody as indicated. (D) HeLa cells transfected with control vector or vector expressing Flag-tagged YY1 in the presence or absence of SET7/9, LSD1 wild type (wt) or enzymatically dead mutant (m) were subjected to IB with anti-YY1K173me1, anti-YY1K411me1, anti-Flag, anti-SET7/9 and anti-LSD1 antibody as indicated. (E) Wild type or SET7/9 knock-out (KO) HeLa cells were transfected with Flag-tagged YY1 followed by immunoblotting (IB) with antibodies as indicated.
