Figure 1: Effects of reoxygenation time on H9c2 cardiomyocytes cell viability and ERS signaling pathways, and the experimental design. | Scientific Reports

Figure 1: Effects of reoxygenation time on H9c2 cardiomyocytes cell viability and ERS signaling pathways, and the experimental design.

From: Cardioprotective effects of Notoginsenoside R1 against ischemia/reperfusion injuries by regulating oxidative stress- and endoplasmic reticulum stress- related signaling pathways

Figure 1

H9c2 cardiomyocytes were subjected to 6 hours of hypoxia and then exposed to different duration (0, 2, 6, 12, 18, 24 h) of reoxygenation. The cell viability was detected using MTT assay, and the cell lyser was analysed by western blotting. (a) Chemical structure of NGR1; (b) Effects of reoxygenation time on H9c2 cardiomyocytes cell viability; (c) Immunoblot analysis of ERS-associated GRP78, P-JNK, JNK, BAD, CHOP, and β-actin were performed in cell lyser; (d) The relative protein expression of GRP78, BAD, CHOP to β-actin, and P-JNK to JNK are expressed in the bar graphs; (e) The experimental design of cultured cardiomyocytes and Langendorff-perfused rat hearts. #P < 0.05 versus the control group, ##P < 0.01 versus the control group, ###P < 0.001 versus the control group.

Back to article page