Figure 4: Effects of NGR1 on I/R-induced heart dysfunction, myocardial cell degeneration, imbalance in redox state, and activated apoptosis pathways in the isolated Langendorff-perfused rat hearts. | Scientific Reports

Figure 4: Effects of NGR1 on I/R-induced heart dysfunction, myocardial cell degeneration, imbalance in redox state, and activated apoptosis pathways in the isolated Langendorff-perfused rat hearts.

From: Cardioprotective effects of Notoginsenoside R1 against ischemia/reperfusion injuries by regulating oxidative stress- and endoplasmic reticulum stress- related signaling pathways

Figure 4

After 15 min of NGR1 or 4-PBA processing, adult rat hearts were subjected to 40 min of global ischemia followed by 60 min of reperfusion. (a) NGR1 showed better effects on improving LVSP, heart rate, + dp/dtmax, and -dp/dt min compared with 4-PBA in the Langendorff I/R model; (b) Histopathological examination showed NGR1’s cardioprotection of the I/R-impaired hearts; (c) The intracellular antioxidant enzyme activity in the isolated rat hearts were examined by measuring MDA, SOD content, and CAT, CK, GSH-Px activities; (d,e) Immunoblot analysis of NGR1’s effects on I/R-induced relative overexpression of apoptosis-associated proteins: P-JNK to JNK, CHOP to β-actin, and Bcl-2 to BAX. #P < 0.05 versus the control group, ##P < 0.01 versus the control group, ###P < 0.001 versus the control group, *P < 0.05 versus the I/R group, **P < 0.01 versus the I/R group, ***P < 0.001 versus the I/R group.

Back to article page