Figure 6: Effects of NGR1 on TM-induced myocardial injury in Langendorff- perfused rat hearts and H9c2 cardiomyocytes.
The hearts with or without 15 min of NGR1 (20 μM) preconditioning were exposed to TM (3 μM) for 15 min, then the solution was replaced by normal perfusate and perfused for another 80 min, which was presented in Fig. 1(e). The normal group was perfused with normal perfusate for 110 min. (a) Effects of NGR1 preconditioning on TM-impaired cardio dysfunction in LVSP, heart rate, + dp/dtmax, and −dp/dtmin; (b) Effects of NGR1 preconditioning on cell viability of H9c2 cardiomyocytes exposed to I/R or TM; (c) TM treatment caused an upregulation of ERS associated proteins, whereas 4-PBA significantly suppressed them; (d) The relative protein expression of GRP78, ATF6, PERK, IRE1, eIf2α, and CHOP to β-actin were represented in bar graph. #P < 0.05 versus the control group, ##P < 0.01 versus the control group, ###P < 0.001 versus the control group, *P < 0.05 versus the I/R group, **P < 0.01 versus the I/R group, ***P < 0.001 versus the I/R group, $P < 0.05 versus the NGR1 + H/R group, ^^P < 0.01 versus the TM group.
