Figure 3: Resveratrol reduces viability of mTOR inhibition sensitive cancer cells.
From: Resveratrol induces autophagy by directly inhibiting mTOR through ATP competition
(A) MCF7 Cells were treated with resveratrol (50 or 100 μM) or PP242 (500 nM) or CCCP (10 μM) for 48 h followed by cell viability measurement by MTT assay. (B) Chemical treatment and viability measurement for SW620 cells were conducted same as MCF7 cells. (C) Intra-cellular signaling was examined in MCF7 cells and SW620 cells after 4 h treatment of resveratrol (50 μM) or PP242 (500 nM). n = 3. (D) MCF7 cells were infected with lenti virus containing vector or shULK1. After 48 h infection chemical treatment was conducted for another 48 h followed by viability measurement. The Y-axis of the graph indicates the levels relative to those of untreated negative control samples.
