Figure 4: Resveratrol inhibits mTOR kinase activity in cell-free systems.
From: Resveratrol induces autophagy by directly inhibiting mTOR through ATP competition
(A) An in vitro mTOR kinase assay was performed with purified mTOR from HEK293 cells. HA-tagged mTOR was introduced into cells and immunoprecipitated with the anti-HA antibody. (B) An in vitro mTOR kinase assay was performed using GST-tagged recombinant mTOR. (C) Inhibition curve of mTOR activity with the indicated concentration of resveratrol. An in vitro kinase assay was performed as in B. n = 3.
