Figure 3

Decrease of mononuclear cell infiltration, activation of hepatic stellate cells (HSC) and inflammation after MLpvNG2⁺ cell transplantation.

(A) H&E staining of naïve, DEN-induced cirrhotic and DEN-induced cirrhotic liver plus MLpvNG2⁺ cell treatment, infiltrating cells indicated within squares (n = 5). Scale bar = 50μm. Immunohistochemistry depicting the presence of F4/80⁺ kupffer cells (Ba–c), F4/80⁺ cells indicated as arrows) (n = 6), α-SMA⁺ HSC (n = 8) (Ca–c) in naive liver (a), DEN-diet liver (b) and DNE plus cell treatment liver (c), α-SMA⁺ cells indicated as squares. Scale bar = 100 μm. (Bd,Cd) Summarized data depicting the % of positive staining within the total cells. Representative blots depicting the level of mRNA expression by RT-PCR(n = 3) (D) and protein expression by western blot analysis (n = 3) (E) for F4⁺/80, TNFα and IL-1β in naïve, DEN-induced cirrhotic liver and DEN-induced cirrhotic liver plus MLpvNG2⁺ cell treatment. DAPI (blue) staining was used to visualize nuclei. Data are shown as mean ± SEM. *P < 0.05, **P < 0.01 DEN⁺ MLpvNG2⁺ cell treated mice vs. DEN⁺ PBS treated mice.