Figure 2: Staining of mouse arterial tissue with directly labeled LO1-750. | Scientific Reports

Figure 2: Staining of mouse arterial tissue with directly labeled LO1-750.

From: Near Infrared Fluorescence (NIRF) Molecular Imaging of Oxidized LDL with an Autoantibody in Experimental Atherosclerosis

Figure 2

(A,B) Direct staining of en face preparations of aortic arches from Ldlr−/− mice, using (A) LO1-750, or (B) isotype control IgG3-750. Staining with the VivoTag-S 750-MAL labeled antibodies is red and nuclei counterstained with SYTO-24 are green. LO1-750 staining was diffuse and concentrated in aggregates at bifurcations of large branches (circles: bct = brachiocephalic trunk, lca = left carotid artery) and on the lesser curvature (lc) (C–H) show representative consecutive serial sections next to the necrotic core of a human carotid atherosclerotic plaque. (D) shows diffuse and focal staining with LO1-750 (red), and (G) shows negative staining with control IgG-750; (C,F) show nuclear counterstaining with SYTO-24 (green); (E,H) are merged images.

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