Figure 8: In vivo targeting with the human-mouse chimeric LO1-Fab-Cys-750. | Scientific Reports

Figure 8: In vivo targeting with the human-mouse chimeric LO1-Fab-Cys-750.

From: Near Infrared Fluorescence (NIRF) Molecular Imaging of Oxidized LDL with an Autoantibody in Experimental Atherosclerosis

Figure 8

(A–B) FMT images 4 hr following iv injection of 50 μg LO1-Fab-Cys-750 (A) or 50 μg of inactivated LO1-Fab-Cys-750 control (B) into litter mate Ldlr−/− mice, fed a HF diet for 42 weeks. LO1-Fab-Cys but not the inactivated control localized to the thoracic ROI; (C) the aorta from (A) was harvested and imaged en face by confocal microscopy. The image shows LO1-Fab-Cys-750 (red) in relation to subendothelial matrix (autofluorescence) (Em 520-590, blue) in the aortic arch. Higher magnification in (D) reveals similar pattern of LO1 localization as seen with whole antibody LO1-750 en face studies ex vivo.

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