Figure 6

SLB mediated Herclon delivery in BT-474 cells.

(A) Confocal images of BT-474 cells incubated with Herclon 633 (46 nM) for 1 hour. Confocal images of BT-474 cells treated with (B) SLB 488-Herclon 633 and (C) CP 488-Herclon 633 for 1 hour. White arrow represents enhanced cytoplasmic localization of SLB 488 and Herclon 633 in (B) and cytoplasmic localization of CP 488 and membrane localization of Herclon 633 in (C). Green = SLB 488/CP 488, red = Herclon 633, blue = DAPI stained nuclei, merge = combination of all three fluorophores and enlarged = enlarged version of merged image. (D) Cell viability assay of BT-474 cells incubated with varying concentration of Herclon (16.6 nM–0.026 nM) with and without SLB (0.166 nM) for 48 hours. (E) Cell viability assay of BT-474 cells incubated with CP (1.66 nM, bar 1) , SLB (1.66 nM, bar 2), Herclon (0.664 nM, bar 3), SLB-Herclon (1:4, bar 4) and CP-Herclon (1:4, bar 5) for 48 hours. Cell viability assay of HeLa cells when incubated with Herclon (0.664 nM, bar 6) and SLB-Herclon (1:4, bar 7), ***p < 0.05