Table 1 Primers used in this study.

From: Intracellular delivery of antibodies by chimeric Sesbania mosaic virus (SeMV) virus like particles

Primer name

Sequence

Length

Description

CP sdm AfeI sen

GCTCTGCTGGATGGGTCGAGCGCTACAGCTGTGGCTGCTGGAC

43

Used for creating AfeI site on SeMV CP βH-βI loop via site directed mutagenesis

CP sdm AfeI anti

CGAGACGACCTACCCAGCTCGCGATGTCGACACCGACGACCTG

43

 

B sen

GATAACAAATTTAACAAAGAACAGC

25

PCR amplification of B domain to be cloned at the βH-βI loop

B anti

TTCTTTCGGCGCCTGCGCAT

20

 

CP sen

ATGGCGAAAAGGCTTTCGAAACAACAG

27

PCR amplification of SLB from pRSETC SLB His for creating pRSETC SLB

CP anti

CAAGAATTCGGTACCTCAGTTGTTCAGGGC

30

 
  1. Sen and anti refers to the sense and antisense primers. The underlined nucleotides in CP sdm AfeI primers refers to the altered nucleotides that replace TTCC in pRSETC CP.
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