Figure 4: Activation of IL-4/STAT6 pathway is in BEAS-2B cells rather than in HFL-1 cells. | Scientific Reports

Figure 4: Activation of IL-4/STAT6 pathway is in BEAS-2B cells rather than in HFL-1 cells.

From: Specific regulation of PRMT1 expression by PIAS1 and RKIP in BEAS-2B epithelia cells and HFL-1 fibroblasts in lung inflammation

Figure 4

The BEAS-2B and HFL-1 cells were incubated with IL-4 (20 ng/mL) for the indicated time, and the expression and phosphorylation of STAT6 and the expression of PIAS1 were detected by Western blotting. The interaction of p-STAT6 and PIAS1 was detected by Co-IP with anti-p-STAT6, anti-PIAS1 antibody or anti-IgG antibody (negative control group) in BEAS-2B and HFL-1 cells with or without IL-4(20 ng/mL) incubated for 24 h (B). The relative mRNA and protein expressions of STAT6 were detected by RT-qPCR and Western blotting after transient transfection with shRNA-STAT6 vector or shRNA-NC vector as control in BEAS-2B cells for 24h (C).The mRNA and protein expression of STAT6 and PRMT1 and phosphorylation of STAT6 in BEAS-2B cells with stable expression of sh2-STAT6 vector and sh-NC vector was detected with or without IL-4 stimulation (D). sh1-STAT6 and sh2-STAT6 vectors were mixed at the ratio of 1:1 to knockdown the STAT6 expression and protein expression of STAT6 and PRMT1 and phosphorylation of STAT6 in BEAS-2B cells was detected with or without IL-4 stimulation (E). Western blotting was shown as representative image and density under the band was measured (ImageJ software) and normalized to β-actin. The results were expressed as mean ± S.E.M of triplicates from three independent experiments and analyzed by One-way ANOVA test. * and ** represent P < 0.05 and P < 0.01 between indicated groups and control group.

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